IBIS (Instituto de Biomedicina de Sevilla) 1 article published in JoVE Developmental Biology 4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy Victor Escrich1, Begoña Ezcurra1, Eva Gómez-Orte1, Cristina Romero-Aranda1, Antonio Miranda-Vizuete2, Juan Cabello1 1CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, 2IBIS (Instituto de Biomedicina de Sevilla), Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain Here, we present a protocol for preparing and mounting Caenorhabditis elegans embryos, recording development under a 4D microscope and tracing cell lineage.