Agriculture and Rural Bureau of Changqing District 1 article published in JoVE Biology Rearing Axenic Delia antiqua with Half-Fermented Sterile Diets Xin Cao1, Qingxia Liang1, Miaomiao Li1, Xiaoqing Wu1, Susu Fan1, Xinjian Zhang1, Fangyuan Zhou1, Zhiqing Zhao2 1Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), 2Agriculture and Rural Bureau of Changqing District A simple procedure for rearing axenic Delia antiqua with half-fermented sterile diets is described. Only one Wolbachia strain was detected in each instar of axenic D. antiqua using PCR.