The Second Affiliated Hospital of Guangzhou Medical University 7 articles published in JoVE Neuroscience Ex Vivo Calcium Imaging for Drosophila Model of Epilepsy Ming-Feng He1, Chu-Qiao Liu2, Xi-Xing Zhang2, Yong-Miao Lin2, Yu-Ling Mao3,4, Jing-Da Qiao1 1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University Here, we present a protocol for ex vivo calcium imaging in GCaMP6-expressing adult Drosophila to monitor epileptiform activities. The protocol provides a valuable tool for investigating ictal events in adult Drosophila through ex vivo calcium imaging, allowing for exploration of the potential mechanisms of epilepsy at the cellular levels. Medicine Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining Jinxing Wei*1, Haixiong Zhang*2, Jinyi Zhong1, Jiyan Xie3, Weibang Yang4, Jinxin Duan1, Wenfeng Zhuo1, Ang Li1, Changzhen Shang1, Jun Min1 1Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, Foshan Fosun Chancheng Hospital, 3Department of Gastrointestinal Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University, 4Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University The present protocol describes fluorescent negative staining in laparoscopic central hepatectomy. This technique can make hepatectomy more accurate and precise. Cancer Research Application of the En Bloc Concept Combined with Anatomic Resection in Laparoscopic Hepatectomy Haopeng Wen*1, Nan Cheng*1, Jiancheng Tang*1, Songhang Liu1, Hongbin Zhou1, Xinyu Li1, Liangqi Cao1 1Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University Numerous studies have demonstrated the advantages of anatomic resection. Nonetheless, whether anatomic resection can increase R0 resection rates remains controversial. Consequently, the present study describes an innovative procedure involving the en bloc concept combined with anatomic resection in laparoscopic hepatectomy, which can reduce postoperative recurrence and metastasis. Medicine Application of Laparoscopic Hepatectomy Combined with Intraoperative Microwave Ablation in Colorectal Cancer Liver Metastasis Dawei Zhang*1, Zuxiao Chen*2, Lei Zhang1, Xiangjun Qian1, Xiaoming Huang1, Zheyu Zheng1, Weidong Pan1 1Department of Pancreatic Hepatobiliary Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University This protocol describes the laparoscopic resection of colorectal cancer liver metastases combined with ultrasound-guided microwave ablation. This technique can safely, effectively, and accurately treat refractory liver metastases <3 cm, reduce postoperative complications, and accelerate the postoperative rehabilitation of patients. Cancer Research Application of Hemostatic Devices in Laparoscopic Hepatectomy Shuian Xie1, Songhang Liu1, Youkui Gao1, Jiancheng Tang1, Liangqi Cao1 1Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University High-grade hemostatic devices are essential for laparoscopic hepatectomy. However, these devices are not generalized in basic medical organizations. Therefore, a suite of simple and easy hemostatic devices is shown in this article, which can make the laparoscopic hepatectomy easier to perform. Neuroscience Massive Pontine Hemorrhage by Dual Injection of Autologous Blood Xiangyue Tang*1, Li Wu*1, Ming Luo1, Zhihua Qiu1, Yongjun Jiang1 1The Second Affiliated Hospital of Guangzhou Medical University We present a protocol to establish a massive pontine hemorrhage model in a rat via dual injection of autologous blood. Neuroscience Establishment of Acute Pontine Infarction in Rats by Electrical Stimulation Ming Luo1, Xiangyue Tang1, Juehua Zhu2, Zhihua Qiu1, Yongjun Jiang1 1The Second Affiliated Hospital of Guangzhou Medical University, 2Department of Neurology, The First Affiliated Hospital of Soochow University Presented here is a protocol for establishing acute pontine infarction in a rat model via electrical stimulation with a single pulse.
