Wuhan University 9 articles published in JoVE Engineering Image Recognition and Parameter Analysis of Concrete Vibration State Based on Support Vector Machine Shuai Wang1,2, Aixun Wang2, Xudong Fu1, Keyang Wu2, Tong Lu2 1Wuhan University, 2Wuhan Construction Engineering Group Co. Ltd. The protocol described in this paper utilizes the directional gradient histogram technique to extract the characteristics of concrete image samples under various vibration states. It employs a support vector machine for machine learning, resulting in an image recognition method with minimal training sample requirements and low computer performance demands. Engineering A Virtual Simulation Experiment of Mechanics: Material Deformation and Failure Based on Scanning Electron Microscopy You Li1, Zhongcheng Lei2, Yong He1, Ze Liu1 1School of Civil Engineering, Wuhan University, 2Department of Artificial Intelligence and Automation, Wuhan University This work presents a three-dimensional virtual simulation experiment for material deformation and failure that provides visualized experimental processes. Through a set of experiments, users can become familiar with the equipment and learn the operations in an immersive and interactive learning environment. Engineering Interactive and Visualized Online Experimentation System for Engineering Education and Research Zhongcheng Lei1, Hong Zhou1, Shengwang Ye1, Wenshan Hu1, Guo-Ping Liu1, Zijie Wei1 1Department of Artificial Intelligence and Automation, Wuhan University This work describes an online experimentation system that provides visualized experiments, including the visualization of theories, concepts, and formulas, visualizing the experimental process with three-dimensional (3-D) virtual test rigs, and visualizing the control and monitoring system using widgets such as charts and cameras. Genetics In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions Lina Yu*1, Wenxiu He*1, Jie Xie*1, Rui Guo*1, Juan Ni2, Xia Zhang3, Quishi Xu1, Caifeng Wang1, Qiuling Yue1, Fangfang Li1, Mengcheng Luo4, Bo Sun3, Lan Ye1, Ke Zheng1 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions. Developmental Biology Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone Jingwen Yang*1,2, Haichun Pan*2, Yuji Mishina2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining. Cancer Research Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues Yuan Wen1, Xie Song-ping2, Liu Pan3, Liu Xiao-yan1, Pan Shan4, Yin Qian4, Sun Meng5, Huang Xiao-xing6, Xiao Rui-jing4, Xiong Jie4, Zhang Qiu-ping4, Shao Liang1 1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results. Bioengineering Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin Kun Lv1,2, Song Gao1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, 2Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University We present a workflow for segmenting and quantifying trabecular bones for 2D and 3D images based on the bone's outer boundary using an ImageJ plugin. This approach is more efficient and accurate than the current manual hand-contouring approach, and provides layer-by-layer quantifications, which are not available in current commercial software. Biology Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM) Jing Men1,2, Jason Jerwick2,3, Penghe Wu1,2, Mingming Chen3,4, Aneesh Alex2,3, Yutao Ma4, Rudolph E. Tanzi5, Airong Li5, Chao Zhou1,2,3 1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images. Bioengineering Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology Xiaofeng Cui*1,2,3, Guifang Gao*2,4, Tomo Yonezawa5,6, Guohao Dai1 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Stemorgan Inc., 3Institute of Advanced Study, Technical University of Munich, 4Institute of Virology, School of Medicine, Wuhan University, 5Department of Molecular and Experimental Medicine, The Scripps Research Institute, 6Research Institute for Biomedical Sciences, Tokyo University of Science The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.