Xiangya Second Hospital of Central South University 4 articles published in JoVE Immunology and Infection Optimization of the Cuff Technique for Murine Heart Transplantation Yunhan Ma*1,2, Baiyi Xie*1,2, Helong Dai*3,4,5, Chenxi Wang6, Shujiao Liu6, Tianshu Lan7, Shuangyue Xu1,2, Guoliang Yan1,2,6, Zhongquan Qi1,2,8 1Organ Transplantation Institute, School of Medicine, Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center, Central South University, 6School of Medicine, Xiamen University, 7Xiamen Medical College, 8Medical College, Guangxi University We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time. Medicine A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice Jie Zhang1,2, Fang Fang1,3, Liang Li1, Haoliang Huang1, Hannah C. Webber1, Yang Sun1,4, Vinit B. Mahajan1,4, Yang Hu1 1Department of Ophthalmology, Stanford University School of Medicine, 2Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 3Department of Ophthalmology, Second Xiangya Hospital of Central South University, 4Department of Ophthalmology, Veterans Affairs Palo Alto Health Care Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal. Biochemistry Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity Meng Zhao1, Yawen Tang1, Patrick J. Ernst1,2, Asher Kahn-Krell1, Chengming Fan1,3, Danielle Pretorius1, Hanxi Zhu1, Xi Lou1, Lufang Zhou2, Jianyi Zhang1, Wuqiang Zhu1 1Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, 3Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model. Medicine Patch Angioplasty in the Rat Aorta or Inferior Vena Cava Hualong Bai1,2,3,4,5, Xin Li6, Takuya Hashimoto1,2,5, Haidi Hu1,2,5, Trenton R. Foster1,2,5, Jesse J. Hanisch1,2,5, Jeans M. Santana1,2,5, Alan Dardik1,2,5 1Department of Surgery, Yale University, 2Vascular Biology and Therapeutics Program, Yale University, 3Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, 4Basic Medical College of Zhengzhou University, 5VA Connecticut Healthcare Systems, West Haven, CT, 6Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation.