In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging (Video) | JoVE

Grace Emin; Sadia T. Islam; Rylee E. King; Velia M. Fowler; Catherine Cheng; Justin Parreno

doi:10.3791/66017

Preparation and Imaging of Murine Ocular Lens Whole Mounts

January 19th, 2024

In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging (Video) | JoVE

01:15 min

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Whole Mount Preparation and Confocal Imaging of the Murine Ocular Lens Anterior Region for Structural, Developmental, and Functional Insights (Video) | JoVE

02:27 min

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Mouse Ocular Lens Equatorial Region Whole Mount Preparation and Confocal Imaging to Understand the Lens Structural-Developmental Relationship (Video) | JoVE

02:12 min

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Grace Emin*¹, Sadia T. Islam*¹, Rylee E. King¹, Velia M. Fowler¹, Catherine Cheng², Justin Parreno^1,3

¹Department of Biological Sciences, University of Delaware, ²School of Optometry and Vision Science Program, Indiana University, ³Department of Biomedical Engineering, University of Delaware

* These authors contributed equally

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The present protocols describe novel whole mount imaging for the visualization of peripheral structures in the ocular lens with methods for image quantification. These protocols can be used in studies to better understand the relationship between lens microscale structures and lens development/function.

Article

Preparation and Imaging of Murine Ocular Lens Whole Mounts

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